Electrophoresis is a technique used to separate and analyze different Molecules, including proteins and DNA, based on their charge and size. It is a powerful tool in molecular biology and biochemistry that is widely used in research, medical diagnosis, and forensic science.
The basic principle of Electrophoresis is to apply an electric field to a mixture of molecules that are suspended in a gel or solution. The electric field causes the molecules to move towards the oppositely charged electrode at different rates, depending on their charge and size. This movement creates a separation of the molecules that can be visualized and analyzed.
The Electrophoresis Market is predicted to reach US$ 2,786.3 Mn in value in 2021 and grow at a CAGR of 5.6% during the forecast period (2021-2028).
One of the most commonly used Electrophoresis techniques is agarose gel electrophoresis. In this technique, a sample of DNA or protein is mixed with a loading buffer and loaded onto a gel made of agarose, a polysaccharide derived from seaweed.
The gel is immersed in a buffer solution that is connected to a power supply. When the electric field is applied, the DNA or protein molecules move through the gel based on their charge and size.
Pharmacogenomics, also known as pharmacogenetics, is a branch of genomic medicine that uses a patient's genetic makeup to customise the medications they receive.
The separation of molecules in agarose gel electrophoresis is based on the size of the molecule. The smaller the molecule, the faster it moves through the gel. The gel is prepared with different concentrations of agarose to create different pore sizes that can separate molecules of different sizes.
A low concentration of agarose creates large pores that allow large molecules to move more easily through the gel, while a high concentration of agarose creates small pores that restrict the movement of large molecules.
After the Electrophoresis is complete, the separated molecules can be visualized using various staining techniques. For DNA, ethidium bromide or SYBR Green can be used to intercalate with the DNA and emit fluorescence when exposed to ultraviolet light. For proteins, Coomassie Blue or silver staining can be used to visualize the protein bands.
Source Link: https://www.prnewswire.com/news-releases/global-electrophoresis-market-to-grow-us-4-291-5-million-by-2028--says-coherent-market-insights-301440954.html
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