Fluid Shifts Dilution Measurements: The 49S subject performed the final Fluid Shifts operations with Dilution Measurements activities.  Saliva, blood and urine samples were collected at multiple points during the day and inserted into a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI).  Fluid Shifts is a joint NASA-Russian experiment divided into Dilution Measurements, Baseline Imaging, and Baseline Imaging with Chibis (Lower Body Negative Pressure). The Fluid Shifts experiment investigates the causes for severe and lasting physical changes to astronaut’s eyes.  Because the headward fluid shift is a hypothesized contributor to these changes, reversing this fluid shift with a lower body negative pressure device is being evaluated as a possible intervention. Results from this study may help to develop preventative measures against lasting changes in vision and to prevention of eye damage.  JEM-Small Satellite Orbital Deployer 7 (J-SSOD 7):  This morning CubeSats from Japan, Ghana, Mongolia, Bangladesh and Nigeria were deployed from J-SSOD 7. These satellites are part of the Joint Global Multi-Nation Birds Satellite project, a cross-border interdisciplinary satellite project for non-space faring countries supported by Japan.  During this 2-year project, students from the Graduate School of Engineering of the Kyushu Institute of Technology design, develop and operate CubeSats belonging to the five participating countries.   Magnetic 3D Cell Culturing:  The crew set up the Magnetic 3D Life Science Hardware in the Microgravity Science Glovebox (MSG) in preparation for the first media change operations.  Cell cultures in space spontaneously grow in three dimensions (3D), which results in characteristics more representative of how cells grow and function in living organisms. But in microgravity, routine manipulation of cell cultures is challenging. Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D Cell Culturing) uses magnetized cells and tools to make it easier to handle cells and cultures, and to improve the reproducibility of experiments. This approach also makes it possible to generate two-dimensional (2D) cultures as controls, and to determine whether biological events in these monolayer cultures result from gravity or substrate attachment.  Rodent Research-5 (RR-5) Systemic Therapy of NELL-1 for Osteoporosis:  The crew refilled water containers in the Animal Habitats for RR-5.  Because spaceflight has significant and rapid effects on the musculoskeletal system it is important to investigate targeted therapies that could ameliorate some of the detrimental effects of spaceflight. The NELL-1 drug being studied in the RR-5 investigation has the potential to slow or reverse bone loss during spaceflight. The Bone Densitometer device measures the density of minerals in bone. Quantitative measures of bone loss in mice during orbital space flight provide data for the development of countermeasures for human crewmembers, as well as for bone-loss syndromes on Earth.  Space Station Remote Manipulator System (SSRMS) Direct Test Drive:  Last night ground teams performed a SSRMS test to gather data on SSRMS joint performance as a baseline for trending joint performance over time.  The test was requested due to a Gearbox Limping (GBL) issue experienced earlier this year which indicated substantial differences between the actual and expected final joint angles. Testing was requested at SSRMS Joint 3 to support the investigation into the root cause of the irregular gearbox twist data by rotating the joint at a consistent rate through the full range of joint travel in both directions on both power strings.  The testing on the first string was completed nominally with no reported issues.  The same test will be performed tonight on the redundant power string.  Today’s Planned Activities All activities were completed unless otherwise noted. Combustion Integrated Rack Alignment Guide Removal Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record Express Rack2 Locker Removal Fluid Shifts Blood 3-Hour Collection – Subject Fluid Shifts Blood Baseline Collection – Subject Fluid Shifts Blood Collection – Operator Fluid Shifts Blood Collection Conclude Stow Fluid Shifts Galley Water Collection Tracer Ingestion Operations – Subject Fluid Shifts MELFI Insertion/Retrieve Fluid Shifts Refrigerated Centrifuge Configuration Fluid Shifts Refrigerated Centrifuge Spin Conclude Fluid Shifts Saliva 3-Hour Collection – Subject Fluid Shifts Saliva 5-Hour Collection – Subject Fluid Shifts Saliva Baseline Collection – Subject Fluid Shifts Urine Collection End – Subject Fluid Shifts Urine Collection Mid – Subject Fluid Shifts Urine Collection – Subject Fluid Shifts Urine Collection Stow Health Maintenance System (HMS) Spaceflight Cognitive Assessment Tool for Windows (WinSCAT) Test Inventory Management System (IMS) conference Long Duration Sorbent Testbed Status Check. Magnetic 3D MSG Life Science Hardware Setup Magnetic 3D Cultures Thaw Systems SABL S/N 3 Insertion MERLIN Relocate Microgravity Measurement Apparatus (MMA) Laptop Terminal 2 Hard Disk Drive (HDD) Exchange Position Return Node 3 to Nominal location after J-SSOD deploy Position Node 3 Camcorder for J-SSOD Deploy. ECLSS Recycle Tank Drain Initiation ECLSS Recycle Tank Drain Termination ECLSS Recycle Tank Fill Part 3 RGN Wastewater Storage Tank Assembly (WSTA) Fill MSPR ELT ethernet Cable Connection/Disconnection Two-Phase Flow Experiment Equipment and Laptop Activation Laptop Setting for Two-Phase Flow (TPF) experiment Water Refill Kit Habitat H2O Fill Water Refill Kit Review Mating Gas Analyzer ГА ГЛ2106 telemetry connectors to БИТС2-12 Test Video Recording for Russia Today TV Channel VIZIR. SM Panels 114 and 116 Outfitting for CКПФ-УМ Payload RADIOSKAF. Activation/Deactivation of Tanusha-SWSU-2 nanosatellite Preparation of Reports for Roscosmos Web Site and Social Media Verification of ИП-1 Flow Sensor Position Installation of Gas Analyzer ГА ГЛ2106 2 Repress from Progress 435 (DC1) СрПК Section 2, initiate/terminate Hose Replacement in СРВ-К2М and Condensate Pump (БПК) R&R Replacement of БСК-1В, Locating equipment TIMER. Battery Charge ECON-M. Observation and Photography Completed Task List Items None Ground Activities All activities were completed unless otherwise noted. J-SSOD CubeSat deploys SSRMS Direct Drive test  QUICK ISS Status – Environmental Control Group:   Component Status Elektron Off Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off          [СКВ] 2 – SM Air Conditioner System (“SKV2”) Off Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate […]