Biotechnology is divided into two words- biology and technology which means we use different techniques to improve our health and environment.
How can we use these techniques???
Answer to this in biotechnology is genetically manipulating microorganisms and for genetically manipulation of microorganisms we need a vector.
Thus, vector is a DNA molecule which is used as a vehicle that carry foreign genetic material which is artificially prepared into another cell. And in another cell it can be replicated or expressed. Vector that contains foreign DNA is called recombinant DNA.
Plasmids are extrachromosomal double-stranded circular DNA molecules. It is found in various bacteria and some eukaryotes.
Vector is artificially manipulated vehicle that carries foreign DNA fragment. It can be plasmid, virus etc.
Human clotting Factor gene could be identified and isolated from the human genome. It is then transferred bacterial plasmid DNA which is then cut open and gene is inserted into the cut DNA.
The inserted gene cloned or expressed in the transgenic (genetically engineered) organism or bacteria. This protein product then isolated and purified from bacteria and injected into hemophilia patients.
Secondly, transfer normal Clotting Factor Gene into the cells producing clotting factor. By expression of gene, protein is produced.
Expression vector is used for the gene expression. The gene we inserted has to be expressed. Expression in simple terms is defined as gene has to produce the protein. First, gene produce mRNA and then mRNA is translated to protein.
The post Hemophilia is an inherited disorder in which the blood clotting mechanism is defective. Because of this defect, people with hemophilia may die from cuts or bruises, especially if internal organs such as the liver, lungs, or kidneys have been damaged. One method of treatment involves injecting a blood-clotting factor that has been purified from blood donations. This factor is a protein encoded by a human gene. Suggest a way in which modern genetic technology could be used to produce this factor on an industrial scale. Is there a way in which the inborn error of hemophilia could be corrected by human gene therapy? appeared first on Questions House.
This post first appeared on Question House - Biology Questions And Answers From Professionals, Students Homework Help, please read the originial post: here