An Autosampler is a guitar that is found in a number of different laboratory applications, especially gas-liquid chromatography, where it is used (as the name implies) to automatically introduce a sample to the inlets of the apparatus being employed in confirmed test. Although it is achievable to manually insert samples with many instruments, that is no further the common practice, since autosamplers provide a better and reproducible method.

Autosamplers might be classified by their capacity, such as for example autosamplers in place of auto-injectors; the latter instrument is effective at running multiple sample at once. Robotic instruments offer another classification of autosampler, with rotating/SCARA robots being among the absolute most widely used.

In gas-liquid chromatography, the column inlet (or injector) makes for the introduction of samples in to a continuous flow of carrier gas. Common inlet types would be the split/splitless injectors, on-column inlets, PTV injectors, the gas source inlet (also called a fuel switching valve), purge and trap systems and SPME (solid phase micro extraction) systems. In the split/splitless injector, the sample is introduced to a heated chamber employing a syringe.

By having an on-column inlet, the sample is introduced in its entirety without the usage of heat. PTV injectors introduce the sample by way of a heated liner at a controlled rate. In the gas source inlet method autosampler, the sample is inserted to the gas stream from collection bottles, a way allowing samples to be introduced without interrupting the carrier gas flow.

Purge and trap autosampler systems involve bubbling an inert gas through aqueous samples, purging insoluble volatile compounds from the matrix. These volatile compounds are then trapped in an absorbent column that is then heated – the volatiles are directed to the carrier stream. Solid phase micro extraction (SPME)is a more economical alternative to purge and trap systems which supplies greater simplicity and a diminished cost.

The sort of automatic sampling system used depends largely on the specific application; in gas chromatography alone, you can find two several types of columns used – with the several types of autosampler being appropriate for one or the other. There are packed columns (usually made of glass or stainless and containing an inert, solid and highly granular material that is coated with a liquid or solid stationary phase).

The other type are capillary columns; these columns feature a really small internal diameter, with the interior of the column being coated with the phase. Other capillary columns are created with a semi-solid construction and parallel micropores; this style allows for great flexibility, so a long column can be wound in to a tight coil which takes up less room.

Although it is gas and liquid chromatography which frequently first spring to mind when discussing several types of autosampler, you can find samplers found in many different applications from the life sciences to geological surveys, the pharmaceutical industry, water quality testing and just about any other application in the materials and life sciences along with quality control testing of types. These instruments allow laboratories to take care of higher sample throughputs while increasing reproducibility and efficiency.

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