There are various methods for extracting cell free DNA from Plasma or serum. One of best is to use kit for extracting cell free DNA. Cfpure is top rated kit for extraction of cell free DNA which is totally pure.
This system uses silica coated magnetic particles for purifying cell free DNA from 1 ml to 10 ml of plasma or serum.
This is less quantity of recovering. For maximizing recovery of cell free DNA for 100 bp to 500 bp DNA fragments, buffers are used.
cfPure kit is totally designed on scalable basis. Scalability meaning in this kit is that you can adjust easily amount of reagents and beads on your need for cfPure™ Cell Free Dna Extraction Kit (100 ml).
Features of cfPure cell free DNA extraction kit:
- Chemical used are totally non toxic
- Automation compatibility is provided
- Cell free DNA recovery is on fastest speed with higher accuracy
- Purified DNA is suitable for PCR, NGS, Bisulfite sequencing etc.
- Provide scalable sample volume input of plasma and serum which is about approx 10 ml
cfPure is one of best quality kit for extraction of DNA. Qualities of cfPure are given below:
- Cost effective: for extracting 1 ml plasma,
- Automation friendly: automated experience is much for cfPure on both pipette based instrument and on King Fisher instrument and happy with you for sharing tips.
- Versatile: for extraction of serum or plasma for 10 ml, bead based system is responsible. This extraction will do in single tubes or 24 to 96 well plates.
- Pure: Cell free DNA which is extracted is suitable for NGS, PCR and in many other applications also.
- Reproducible: beads, protocols and chemistry are used for getting appropriate results.
Magnetic Bead Based Protocol Which Is Efficient And Rapid:
Protocol, which is used for cfPure is easy and rapid.
This bead based protocol allows users to check out number of samples in 60 minutes or in less time. All such checked samples are ideal for biomarker screening. BioChain kit has developed one technology which is used during isolation.
Technology, named as silica coated magnetic bead, and developed to find out cell free DNA in isolation process which has low molecular weight.
If you want to purify small amount of plasma, then use less magnetic bead solution. Purifying small amount of plasma is easy to perform and easy to automate.
This less purification saves your money.
Cell free DNA is used in early detection of cancer and also in prenatal genetic testing.
Optimized system which is using magnetic bead protocol provide facility to user for using protocol with different volume of samples which varies from 100 ml to 10 ml. this varying range of sample give best reproducible results. cfPure cell free DNA kit is available in two varying sizes.
One kit used 100 ml human plasma for extraction cell free DNA with enough reagents while other kit is larger than this and used 100 ml human plasma which consist sufficient reagents for extraction.
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