When growing Microorganisms, several conditions including incubation time, temperature, pH, adequate moisture and sufficient nutrients (such as Carbon and nitrogen) must be considered to ensure successful results.
Microbiologics have an interesting Paper on those organisms that require a Carbon Dioxide rich atmosphere and the paper provides tips for cultivating these organisms using chocolate agar.
To access the paper, see Microbiologics
Posted by Dr. Tim Sandle
Pharmaceutical Microbiology (c) Dr Tim Sandle http://www.pharmamicroresources.com