The Court of Arbitration for Sport has dismissed an appeal by 47 Russian Athletes and coaches against a ban on participation in the Winter Olympics.
Russian Deputy Prime Minister Vitaly Mutko said the decision was understandable but disappointing.
It comes amid a long-running row over Russian doping which has seen the country banned from the games.
However 169 Russians will compete as independents.
The CAS decision was made just hours before Friday's opening ceremony.