From March, the supermarket will ask customers to prove they are over the age of 16 if they want to buy the highly caffeinated beverages.
The decision follows similar moves from Waitrose, Sainsbury's, Aldi, Asda, Morrisons and Lidl in the past few weeks.
Tesco spokesperson Jane Lawrie said: "With millions of customers across the UK shopping with us every week and 300,000 colleagues working in our stores, centres and offices, implementing new measures on energy drinks is another opportunity to help our customers adopt healthy habits.
"We know that this isn't easy to implement, so in addition, we will create awareness in store and online to highlight that high-energy caffeine drinks are not recommended for children, supporting the #notforchildren campaign."
The decision comes after a study in the BMJ Open journal which prompted experts to call for a nationwide ban on the sale of energy drinks to under 16s.