Crews have finished their deep-sea search of a stretch of the Indian Ocean without finding a single trace of the Boeing 777, which was travelling from Kuala Lumpur to Beijing in March 2014 when it disappeared.
There were 239 people on board, including 12 members of crew. Most of the passengers were from China.
The Joint Agency Co-ordination Centre said the £133m search had been officially suspended after crews finished their fruitless search of the 46,000-square mile search zone west of Australia.
The end of the hunt means the world's greatest aviation mystery may never be solved.
In July 2015 part of the aeroplane's wing was found on Reunion Island, and so far more than 20 objects either confirmed or believed to be from the jet have washed ashore on beaches in Mauritius, Mozambique, Madagascar and Tanzania.
But while the debris confirms the plane went down in the Indian Ocean, the location of the main wreckage and what happened to MH370 remain a mystery.
A number of theories have been put forward, including a fire on board, hijacking and pilot suicide.
In a joint statement the transport ministers of Malaysia, Australia and China said: "Despite every effort using the best science available, cutting edge technology, as well as modelling and advice from highly skilled professionals who are the best in their field, unfortunately, the search has not been able to locate the aircraft.
"Accordingly, the underwater search for MH370 has been suspended. The decision to suspend the underwater search has not been taken lightly nor without sadness."
The families of those on board have expressed their dismay at the decision, and called on the authorities to reconsider.